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Table 1 The sensitivity of LAMP-BART, conventional LAMP, and 3M MDA Salmonella in spiked food and feed samples based on three independent repeats

From: Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time

Food or feed type

Salmonella serovar

Detection limit (CFU/25 g) a-b without enrichment

LAMP-BART

Conventional LAMP

3M MDA Salmonella

Food samples

 Cantaloupe

Poona

2.0 × 104

2.0 × 104

2.0 × 105

 Ground beef

Typhimurium

2.0 × 105b

2.0 × 105a

2.0 × 106a

 Ground turkey

Heidelberg

1.7 × 105

1.7 × 105

1.7 × 108

 Shell eggs

Enteritidis

1.7 × 104

1.7 × 104

1.7 × 105b

 Tomato

Typhimurium

2.0 × 104

2.0 × 104

2.0 × 105a

Feed samples

 Cattle feed

Newport

1.7 × 105

1.7 × 105

1.7 × 106

 Chicken feed

Enteritidis

1.7 × 106b

1.7 × 106b

1.7 × 106a

 Dry dog food

Infantis

1.1 × 105

1.1 × 105

1.1 × 106a

  1. a-bIn one (a) or two (b) out of three repeats, the assays detected respective Salmonella serovars at concentrations 10-folder lower than those presented
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BMC Microbiology

ISSN: 1471-2180

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