Table 2 Analytical sensitivities of N-PCR and Q-PCR using DNA extracted from different dilutions of S. Typhi cultures and spiked blood samples
From: Evaluation of fliC-d based direct blood PCR assays for typhoid diagnosis
Dilution factor | Organisms/ml | Result determined by | |||
|---|---|---|---|---|---|
Bacterial culture | Spiked blood | ||||
N-PCR | Q-PCR (Cta) | N-PCR | Q-PCR (Cta) | ||
Stock | 4.2 × 108 | + | + (16.0) | NA | NA |
10−1 | 4.2 × 107 | + | + (19.1) | + | + (19.5) |
10−2 | 4.2 × 106 | + | + (22.5) | + | + (23.1) |
10−3 | 4.2 × 105 | + | + (25.7) | + | + (26.2) |
10−4 | 4.2 × 104 | + | + (28.2) | + | + (29.8) |
10−5 | 4.2 × 103 | + | + (31.1) | + | + (33.1) |
10−6 | 4.2 × 102 | + | + (33.6) | + | + (36.5) |
10−7 | 4.2 × 101 | + | + (36.9) | - | - (39.2) |
10−8 | 4.2 × 100 | - | - (40.0) | - | - (40.0) |