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Table 1 Bacterial strains used in this study

From: Evaluation of fliC-d based direct blood PCR assays for typhoid diagnosis

Sl. no.

Name of organism

Strain/sample ID

Result determined by

C-PCR

N-PCR

Q-PCR (Cta)

1

Salmonella Typhi

MTCC 734

+

+

+ (19.1)

2

Salmonella Typhi

KOL 38

+

+

+ (20.0)

3

Salmonella Paratyphi A

KOL 24

-

-

- (40.0)

4

Salmonella Paratyphi A

MTCC 735

-

-

- (40.0)

5

Salmonella Typhimurium

NCTC 74

-

-

- (40.0)

6

Salmonella Typhimurium

BCH 7332

-

-

- (40.0)

7

Salmonella Enteritidis

BCH 7321

-

-

- (40.0)

8

Salmonella Enteritidis

EVS 111

-

-

- (40.0)

9

Salmonella Worthington

BCH 3008

-

-

- (37.0)

10

Salmonella Worthington

BCH 2770

-

-

- (40.0)

11

Salmonella Weltrevreden

OSS 56

-

-

- (38.5)

12

Salmonella Weltrevreden

OSS 57

-

-

- (40.0)

13

Salmonella Kentucky

EVS 318

-

-

- (40.0)

14

Salmonella Kentucky

EVS 319

-

-

- (40.0)

15

Salmonella Bareilly

EVS 44

-

-

- (36.9)

16

Salmonella Bareilly

EVS 45

-

-

- (39.1)

17

Salmonella Idikan

EVS 30

-

-

- (40.0)

18

Salmonella Idikan

EVS 31

-

-

- (38.4)

19

Salmonella Senftenberg

EVS 100

-

-

- (36.2)

20

Salmonella Virchow

EVS 160

-

-

- (36.7)

21

Escherichia coli

ATCC 35218

-

-

- (40.0)

22

Escherichia coli

U 1953

-

-

- (40.0)

23

Escherichia coli

U 2367

-

-

- (40.0)

24

Escherichia coli

U 2368

-

-

- (40.0)

25

Escherichia coli

BT 68

-

-

- (40.0)

26

Escherichia coli

BT 171

-

-

- (40.0)

27

Klebsiella pneumonia

U 1791

-

-

- (40.0)

28

Klebsiella pneumonia

U 1947

-

-

- (40.0)

29

Klebsiella sp.

P 1837

-

-

- (40.0)

30

Klebsiella sp.

P 1836

-

-

- (40.0)

31

Klebsiella sp.

P 1745

-

-

- (37.2)

32

Acinetobacter sp.

P 1872

-

-

- (40.0)

33

Acinetobacter sp.

JN 27

-

-

- (40.0)

34

Acinetobacter sp.

SP 2

-

-

- (38.2)

35

Acinetobacter sp.

BCR 154

-

-

- (40.0)

36

Acinetobacter sp.

BCR 188

-

-

- (40.0)

37

Shigella flexneri 2a

BCH 7286

-

-

- (38.2)

38

Shigella dysenteriae

BCH 5375

-

-

- (40.0)

39

Shigella boydii

BCH 4087

-

-

- (40.0)

40

Shigella sonnei

BCH 7178

-

-

- (40.0)

41

Citrobacter freundii

NTS 63

-

-

- (40.0)

  1. C-PCR, conventional PCR; N-PCR, nested PCR; Q-PCR, real-time quantitative PCR; Ct, cycle threshold
  2. aThe Ct cutoff value for positive result is ≤30