Table 7 Linoleic acid metabolism (mg/tube) in presence of 22:6n-3 by B. proteoclasticus P18. Incubation was performed in growth medium containing 40 μg/mL (0.4 mg/tube) of 18:2n-6 in presence of 0, 10 μg/mL (Low: 0.1 mg/tube) and 40 μg/mL (High: 0.4 mg/tube) 22:6n-3. Growth medium included 50 % (v/v) of autoclaved-uncentrifuged rumen fluid and H₂ gas was the headspace gas. Value-presented in this table included the fatty acids and VFA of autoclaved-uncentrifuged rumen fluid used to prepare the growth medium

18:2n-6

0

0.08 ± 0.009^bc

0.08 ± 0.002^bc

0.09 ± 0.012^b

0.06 ± 0.001^c

0.05 ± 0.002^c

(mg/tube)

Low

0.09 ± 0.002^bc

0.09 ± 0.001^bc

0.10 ± 0.003^b

0.07 ± 0.006^c

0.06 ± 0.002^c

High

0.10 ± 0.011^bc

0.10 ± 0.004^b

0.10 ± 0.006^b

0.07 ± 0.006^c

0.06 ± 0.005^c

CLA

0

0.00^b

0.00^b

0.00^b

0.00^b

0.00^b

(mg/tube)

Low

0.00^b

0.00^b

0.00^b

0.00^b

0.00^b

High

0.00^b

0.00^b

0.00^b

0.00^b

0.00^b

VA

0

0.21 ± 0.011^c

0.07 ± 0.003^d

0.06 ± 0.005^d

0.06 ± 0.003^d

0.04 ± 0.008^a

(mg/tube)

Low

0.29 ± 0.015^c*

0.10 ± 0.007^d*

0.07 ± 0.003^e

0.07 ± 0.004^e

0.07 ± 0.004^e*

High

0.28 ± 0.005^b*

0.19 ± 0.005^c**

0.11 ± 0.008^d*

0.13 ± 0.010^d*

0.10 ± 0.002^d**

18:0

0

0.58 ± 0.014^b

0.74 ± 0.056^c

0.73 ± 0.020^c

0.71 ± 0.016^c

0.73 ± 0.019^c

(mg/tube)

Low

0.53 ± 0.022^a*

0.67 ± 0.019^b*

0.69 ± 0.018^bc*

0.72 ± 0.010^c

0.73 ± 0.010^c

High

0.52 ± 0.017^a*

0.54 ± 0.015^a**

0.58 ± 0.015^b**

0.65 ± 0.017^c*

0.68 ± 0.007^c*

VFA

0

469.6 ± 16.80^b

541.7 ± 12.61^c

545.7 ± 8.57^c

585.2 ± 30.61^d

567.6 ± 15.93^d

(μmol/tube)

Low

460.6 ± 3.09^b

527.9 ± 6.54^cd

542.9 ± 4.34^de

557.0 ± 7.23^e*

551.4 ± 5.35^e

High

468.5 ± 21.71^b

531.8 ± 8.25^c

549.7 ± 6.14^cd

562.1 ± 17.97^d*

559.4 ± 2.82^d

22:6n-3

Low

0.06 ± 0.002^b

0.02 ± 0.001^c

0.02 ± 0.001^c

0.02 ± 0.003^c

0.01 ± 0.000^d

(mg/tube)

High

0.29 ± 0.011^a

0.17 ± 0.002^b

0.11 ± 0.004^c

0.09 ± 0.012^c

0.06 ± 0.006^d