Table 6 Amount of metabolized 22:6n-3 (μg/tube) recovered in FAME peaks. Incubation was performed in growth medium containing autoclaved-uncentrifuged rumen fluid (50 % v/v) in the presence of 22:6n-3 (5, 10, 20, 40 and 80 μg/mL) with Butyrivibrio proteoclasticus P18. Tubes were withdrawn at 48 h of incubation to study the extent of 22:6n-3 biohydrogenation. Peak numbers correspond to peaks in Fig. 2

1

Δ7, Δ10, Δ13, Δ16, Δ19-22:5

0.63 ± 0.75

1.15 ± 0.28

2.93 ± 1.04

25.14 ± 3.03

77.118 ± 1.30

2

Δ4, Δ10, Δ13, Δ16, Δ19-22:5

-

-

0.43 ± 0.58

5.08 ± 1.05

21.42 ± 0.69

3

Δ5, Δ10, Δ13, Δ16, Δ19-22:5

0.87 ± 1.13

1.11 ± 0.96

3.68 ± 2.42

29.16 ± 3.92

177.29 ± 5.04

4

Δ10, Δ13, Δ16, Δ19-22:4

5.26 ± 1.98

8.14 ± 2.11

19.69 ± 2.41

208.41 ± 14.7

78.81 ± 7.61

5

Δ13, Δ16, Δ20-22:3

0.41 ± 0.82

1.17 ± 0.82

3.63 ± 1.62

15.80 ± 2.34

12.05 ± 1.17

6

Δ13, Δ16, Δ19-22:3

5.82 ± 0.77

14.26 ± 0.62

36.02 ± 5.88

22.22 ± 3.63

3.48 ± 1.19

7

Δ13, Δ16, Δ20-22:3

2.84 ± 0.46

5.02 ± 0.20

7.24 ± 0.49

-

-

8

Δ11, Δ16, Δ19-22:3

2.33 ± 0.86

4.26 ± 0.66

8.77 ± 1.22

26.98 ± 3.47

5.61 ± 0.81

9

Δ16, Δ20-22:2

4.14 ± 0.53

7.14 ± 0.39

10.75 ± 0.76

-

-

10

Δ16, Δ19-22:2

7.81 ± 0.81

15.92 ± 0.87

37.31 ± 4.61

5.53 ± 1.24

3.09 ± 1.05

11

Δ16, Δ20-22:2

8.83 ± 0.63

12.94 ± 0.59

16.69 ± 1.46

-

-

12

Δ14, Δ18-22:2

3.34 ± 0.65

5.09 ± 0.35

7.49 ± 0.56

-

-