Table 4 Central Composite Design in coded units
Run | Initial pH (A) | Temperature (B) | Peptone (C) | MgSO4.7H2O (D) | Metabolite yield (mg/l) |
|---|---|---|---|---|---|
1 | 0 | 0 | 0 | 0 | 217.56 |
2 | −1 | 1 | −1 | −1 | 70.34 |
3 | 0 | 0 | 0 | 2 | 67.8 |
4 | −1 | 1 | −1 | 1 | 73.78 |
5 | 1 | −1 | 1 | −1 | 73.78 |
6 | 1 | 1 | −1 | 1 | 55 |
7 | 0 | 0 | 0 | 0 | 210.7 |
8 | 1 | 1 | 1 | 1 | 32.15 |
9 | 0 | −2 | 0 | 0 | 71.32 |
10 | 0 | 0 | 0 | 0 | 210.65 |
11 | 0 | 0 | 0 | 0 | 217.5 |
12 | 1 | 1 | −1 | −1 | 66.2 |
13 | −1 | −1 | 1 | −1 | 77.7 |
14 | 0 | 0 | 2 | 0 | 80.25 |
15 | 1 | −1 | 1 | 1 | 75.2 |
16 | 0 | 0 | 0 | 0 | 205.5 |
17 | 1 | 1 | 1 | −1 | 75 |
18 | 1 | −1 | −1 | 1 | 80.14 |
19 | −1 | 1 | 1 | 1 | 62 |
20 | 2 | 0 | 0 | 0 | 80 |
21 | −1 | −1 | 1 | 1 | 66.5 |
22 | −1 | −1 | −1 | −1 | 78.5 |
23 | 0 | 0 | 0 | 0 | 205.46 |
24 | 0 | 0 | 0 | −2 | 77.58 |
25 | 0 | 0 | −2 | 0 | 51.3 |
26 | −1 | −1 | −1 | 1 | 70.85 |
27 | 1 | −1 | −1 | −1 | 46.76 |
28 | 0 | 2 | 0 | 0 | 48 |
29 | −2 | 0 | 0 | 0 | 70 |
30 | −1 | 1 | 1 | −1 | 78.45 |