Transcriptomic and proteomic analyses of core metabolism in Clostridium termitidis CT1112 during growth on α-cellulose, xylan, cellobiose and xylose

Table 4 Znet-scores (normalized differential values) of C. termitidis genes encoding putative hydrogenases

		Znet-scores				Znet-scores
Locus	Locus-description	R0	P0	R1	P1	R2	P2
NiFe H₂ase
Cter_0657	Ni,Fe-hydrogenase III large subunit	0.12	−0.25	−0.68	−1.13	−0.05	−0.74
Cter_3893	Ni,Fe-hydrogenase III large subunit	−0.03	0.71	−1.19	−0.31	0.06	0.25
Cter_3894	Respiratory-chain NADH dehydrogenase, 30 Kd	0.19	0.91	−1.27	0.24	0.3	1.01
Cter_3895	Ni,Fe-hydrogenase III small subunit	−0.02	0.56	−1.32	0.01	0.12	0.52
FeFe H₂ase
Cter_4761	Hydrogenase, Fe-only	−0.56		−0.6		−0.91
Cter_4762	NADH:ubiquinone oxidoreductase, NADH-binding (51 kD)	−0.38	−0.64	−0.41	−0.75	−0.39	−0.23
Cter_4763	Ferredoxin	−0.11		−0.46		1.03
Cter_4765	NADH-quinone oxidoreductase, E subunit	−1.01		−0.25		−0.19
Cter_4848	Hydrogenase, Fe-only	0.18	−0.24	−0.94	−0.87	−0.98	−0.1
Cter_4849	NADH:ubiquinone oxidoreductase, NADH-binding (51 kD)	0.06	−0.25	−1.07	−0.82	−1.2	−0.13
Cter_4850	NADH:ubiquinone oxidoreductase 24 kD subunit	0.03	−0.22	−1.44	−0.88	−0.98	−0.24
Cter_2461	Hydrogenase, Fe-only	0.34		0.52		0.23
Cter_2462	NADH:ubiquinone oxidoreductase, NADH-binding (51 kD)	0.05		−0.19		−0.06
Cter_2463	NADH:ubiquinone oxidoreductase 24 kD subunit	−0.05		−0.04		−0.11
Cter_2553	Iron only hydrogenase large subunit	−0.1	0.62	−0.13	0.61	−0.67	0.26
Cter_3528	Iron only hydrogenase large subunit	0.5		0.04		0.25
Cter_4457	Hydrogenase, Fe-only	0.69		0.81		−0.01
Cter_4458	NADH:ubiquinone oxidoreductase, NADH-binding (51 kD)	0.19		1.07		0.27
Cter_4459	NADH:ubiquinone oxidoreductase 24 kD subunit	0.18		0.46		−0.27

Normalized transcriptomic Z-scores Rnet are represented as: (i) R0: xylose grown cells – cellobiose grown cells; (ii) R1: α-cellulose grown cells – cellobiose grown cells; and (iii) R2: xylan grown cells – cellobiose grown cells. Normalized proteomic Z-scores Pnet are represented as (i) P0: xylose grown cells – cellobiose grown cells; (ii) P1: α-cellulose grown cells – cellobiose grown cells; and (iii) P2: xylan grown cells – cellobiose grown cells. For any protein or RNA transcripts, a negative value represents higher expression on cellobiose, while a positive value represents higher expression in any of the other corresponding substrate. Bold indicates the bifurcating hydrogenase abundantly expressed under all conditions

ISSN: 1471-2180