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Fig. 4 | BMC Microbiology

Fig. 4

From: The influence of a modified lipopolysaccharide O-antigen on the biosynthesis of xanthan in Xanthomonas campestris pv. campestris B100

Fig. 4

MALDI-TOF-MS analysis of the LPS O-antigen of Xanthomonas campestris pv. campestris B100 wild type, the O-antigen branch mutants Xcc H28110 (wxcK) and Xcc H20110 (wxcN) and O-antigen deficient mutant Xcc H21012 (wxcB). Spectra related to the mutants Xcc H28110 (wxcK) and Xcc H20110 (wxcN) to the B100 wild type O-antigen were compared in order to verify and deeper analyze the inhibition of the OA side branches and the increase in rhamnose moieties. Displayed are ranges of the spectra with a focus on the O-antigen constituents, rhamnose (rha) and N-acetylfucosamine (FucNAc). Examples of repeating peaks are marked. No OA residues were detected in the LPS of H21012 (wxcB), but we could measure different lipid A moieties and a peak representing the rough-type LPS. Measurements were carried out with 5 μg LPS of each strain

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BMC Microbiology

ISSN: 1471-2180

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