Fig. 3

Analysis of the LPS O-antigen of Xanthomonas campestris pv. campestris B100 wild type and three mutant strains by gas chromatography following methanolysis. Wild type data are compared to the wxcB mutant H21012, wxcK mutant H28110, and wxcN mutant H20110. a Complete chromatogram with the O-antigen main constituent rhamnose (Rha) annotated followed by a box indicating signals related to the second O-antigen constituent N-acetylfucosamine (FucNAc) and LPS core moieties. b Zoom-in of the boxed area of the chromatogram with peaks identified to represent the O-antigen constituent FucNAc and the LPS core constituent galacturonic acid (GalA), which are both represented by two peaks. Measurements were carried out after LPS isolation applying the hot phenol / water method followed by methanolysis with 0.5 M HCl in methanol and the respective peracetylation of 100 μg LPS