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Fig. 1 | BMC Microbiology

Fig. 1

From: The influence of a modified lipopolysaccharide O-antigen on the biosynthesis of xanthan in Xanthomonas campestris pv. campestris B100

Fig. 1

Gene region involved in the LPS O-antigen biosynthesis in Xanthomonas campestris pv. campestris B100. a Mutant names (Xcc H21012 (wxcB), Xcc H28110 (wxcK) and Xcc H20110 (wxcN)) and positions of Tn5 insertions in the Xcc the genome are marked [14, 22]. The flag indicates the orientation of the lacZ reading frame. b Restriction sites of endonucleases are given (B: BamHI; E: EcoRI, H: HindIII; X: XhoI). c Detailed map of the wxc gene cluster with the respective gene names. Mutated genes are highlighted in grey. d Functional organization of the gene cluster following the analyses of Vorhölter and colleagues [14]: Region 1 is supposed to be involved in the biosynthesis of the poly-rhamnan main chain, region 2 plays a role in the nucleotide sugars biosynthesis leading to GDP-rhamnose. Region 3 is putatively responsible for the N-acetylfucosamine branches of the Xcc OA

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