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Fig. 5 | BMC Microbiology

Fig. 5

From: Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety

Fig. 5

Influence of host genotype on symptoms, pathogenesis and qPCR results. Comparison of pathogenesis of the wild type reference strain on four maize varieties. a) Representative infection sites as observed at the indicated time points. b) Quantitation of fungal morphogenesis and host defence reactions. Columns give means of four biological replicates performed in different weeks, each in three technical replicates representing different leaves. On each leaf, infection structures developing from 100 conidia and the corresponding host responses were counted. Error bars provide standard errors. Letters assess variation between host varieties at 2 dpi, which was determined by multiple comparisons using the Student-Newman-Keuls test. Different letters indicate significant differences at P < 0.05. c) Fluorescence microscopy using UV2A filter exhibits whitish papillae and dark appressoria (600×). Arrows indicate papillae. Bars represent 50 μm. d) Fluorescence microscopy using UV2A filter and autowhite function on DAB-stained tissues exhibits sites of hydrogen peroxide production (200×). Results from the other two varieties were similar to those shown. Bars represent 100 μm. e) Results of qPCR using 10 ng of total DNA as template. Each column represents the absolute amount of fungal DNA that was averaged from five leaf disc pools. Each pool comprised eight leaf discs excised from individual leaves carrying a single inoculation site at their middle. Error bars indicate standard errors. Letters assess variation between host varieties at a given time as determined by the Student-Newman-Keuls test. Different letters indicate significant differences at P < 0.05

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