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Fig. 3 | BMC Microbiology

Fig. 3

From: Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety

Fig. 3

Effects of mutations affecting virulence on qPCR results. Three ATMT mutants (AT-1, AT-2 and AT-3) were inoculated on maize variety Mikado and compared to the wild type reference. a Symptoms occurring at 120 hpi. b Light microscopy of infection sites after staining with aniline blue. Arrowheads indicate appressoria, arrows invasive secondary hyphae and asterisks acervuli. Photographs shown in a and b provide representative results from five repeated experiments each using four leaves per variant. c Result of qPCR using 10 ng of total DNA as template. Columns represent means of five independent experiments. For each experiment, eight leaf discs excised from individual leaves carrying a single inoculation site at their middle were pooled. Error bars indicate standard errors. Letters assess variation between the strains at a given time, which was determined by multiple comparisons using the Student-Newman-Keuls test. Different letters indicate significant differences at P < 0.05

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