Fig. 7From: Biochemical and spectroscopic characterization of purified Latex Clearing Protein (Lcp) from newly isolated rubber degrading Rhodococcus rhodochrous strain RPK1 reveals novel properties of LcpInhibition of LcpRr by potential inhibitors. Activity was determined using the HPLC-based detection of polyisoprene cleavage products after 2 h of incubation of polyisoprene latex with Lcp at room temperature and subsequent solvent extraction of the products. The final concentration of inhibitors was 1 mM. The 100 % value corresponded to the area of the C35 (23 min) product peak (see Fig. 3b). Sensitivity to carbon monoxide was tested by replacing 0.5 volumes of (oxygenated) assay buffer by a deoxygenated and CO-saturated assay buffer. Cleavage products were solvent-extracted after 2 h of incubation time. At least two repetitions with two technical replicates were performed for each inhibitor. Error bars indicate the upper and lower values of one experimentBack to article page