Fig. 1

HrcU in Erwinia amylovora. a Schematic representation of HrcU domain organization. Letters indicate predicted transmembrane domains (TM), the plasmamembrane (PM) and the cytoplasm (CP). Numbers denote amino acid positions based on the genome sequence of E. amylovora ATCC 49946 (NCBI NC_013971). The NPTH motif is labeled in red and the arrow represents the site of cleavage and conformational change reported in homologous proteins. b T-coffee multiple sequence alignment of C-terminal NPTH motif in HrcU homologs. Weblogo was used to visualize an alignment of HrcU homologs from animal pathogens Yersinia enterocolitica, Shigella flexneri and Escherichia coli, plant pathogens Erwinia amylovora, Xanthomonas campestris and Pseudomonas syringae as well as a flagellar homolog from Salmonella enteric. NPTH is conserved in all HrcU homologs