Target identification in Fusobacterium nucleatum by subtractive genomics approach and enrichment analysis of host-pathogen protein-protein interactions

Table 1 Metabolic pathway-independent druggable proteins

S. No.	Gene	Protein name	Uniprot ID	Function
1	FN1275	Acriflavin resistance protein B	Q8RE51	Resistant to aminoglycosides such as amikacin, gentamycin, kanamycin, neomycin and tobromycin as well as to amphiphilic compounds. Thus, inhibiting AcrB function may lead to pathogen’s susceptibility to these antibiotics.
2	FN1321	Acetoacetate metabolism regulatory protein atoC	Q8RE11	Required for catabolism of short-chain fatty acids. Plays role in flagella synthesis, sodium (but not potassium) sensitivity and chemotaxis.
3	ftsZ	Cell division protein FtsZ	Q8RDQ7	Required for proper localization of division plane during bacterial cytokinesis. Inhibition of its GTP-dependent polymerization activity prevents cells to divide
4	FN0724	Flavodoxin	Q8RFH4	Acts as an electron acceptor of pyruvate-oxidoreductase complex. Flavodoxin inhibitors were reported to act against Helicobacter pylori’s infection that causes gastritis or chronic peptic ulcers
5	FN0756	Pseudouridine synthase	Q8RFE9	Catalyze post-transcriptional modifications of cellular RNAs i.e., site-specific isomerisation of uridine.
6	rsgA	Putative ribosome biogenesis GTPase RsgA	Q8R685	Involved in 30S ribosome subunit biogenesis.
7	FN0809	Putative tRNA (cytidine(34)-2’-O)-methyltransferase	Q8R673	Methylates 2’-O-ribose group of cytidine present at 34^thposition of tRNA anticodon loop.
8	sigA	RNA polymerase sigma factor SigA	Q8RE13	Essential for survival of bacteria as it regulates transcription of housekeeping promoters.