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Table 1 Genes involved in the acyclic terpene utilization (Atu) and leucin isovalerate utilization (Liu) pathway

From: The anaerobic linalool metabolism in Thauera linaloolentis 47 Lol

Enzyme

NCBI accession

Length [AA]

AA similarity [%]

E-value

Protein identification by MS

Linalool isomerasea

ENO87364

644

20

3E-10

Yes

Geraniol dehydrogenasea (GeoA)

ENO84122

366

46

7E-96

Yes

Geranial dehydrogenasea (GeoB)

ENO84123

456

31

7E-39

No

Acyl-CoA synthaseb (AtuH)

ENO87356

545

24

1E-15

No

Geranyl-CoA carboxylaseb beta-subunit (AtuC)

ENO87361

545

54

0

Yes

Geranyl-CoA carboxylaseb alpha subunit (AtuF)

ENO87362

705

52

0

Yes

Isohexenyl-glutaconyl-CoA hydrataseb (AtuE)

ENO87363

258

30

7E-27

No

3-Hydroxy-3-isohexenylglutaryl-CoA:acetate lyaseb (AtuA)

ENO84124

615

52

0

Yes

3-Methylcrotonyl-CoA carboxylaseb beta-subunit (LiuB)

ENO88226

535

72

0

Yes

3-Methylcrotonyl-CoA carboxylaseb alpha subunit (LiuD)

ENO88223

668

53

0

Yes

3-Methylglutaconyl-CoA hydrataseb (LiuC)

ENO88225

265

44

3E-73

No

Hydroxymethylglutaryl-CoA lyaseb (LiuE)

ENO88221

312

63

2E-129

No

  1. Genes were identified by amino acids alignments using C. defragrans 65Phena and P. aeruginosa PAO1b sequences as references. Proteins identified in the proteomic approach were analyzed by SDS-PAGE coupled to MALDI-ToF MS (Additional file 2: Figure S1)