Fig. 5From: Optimized methods for Legionella pneumophila release from its Acanthamoeba hostsEffectiveness of the ISO 11731:1998 method in the recovery of L. pneumophila Paris and Olda and Acanthamoeba SPA08 and 1BU in a free-living state and in co-culture. Bacterial inactivation was determined using viable counts on supplemented BCYE agar medium, and amoebal inactivation was determined using the MPN method. Data are presented as the means ± SD (columns and error bars)Back to article page