Fig. 4
From: Optimized methods for Legionella pneumophila release from its Acanthamoeba hosts
Efficiency of intracellular growth of L. pneumophila Paris and Olda within Acanthamoeba SPA08 and 1BU at different time points. Bacterial and amoebae cells were stained using the MONOFLUO™ Legionella pneumophila IFA Test Kit and counted using an epifluorescent microscope. Amoebae were divided in four different categories to describe their state: not-infected, low-infected (< 10 L. pneumophila/amoeba, early stage of infection), medium-infected (quantifiable replicating L. pneumophila cells) and high-infected (non-quantifiable, amoebae full of L. pneumophila cells with no cytosol left). Data are presented as the percentage ± SD (columns and error bars)