Fig. 3
From: Optimized methods for Legionella pneumophila release from its Acanthamoeba hosts
Intracellular growth of L. pneumophila strains within Acanthamoeba strains. Pictures were obtained using the MONOFLUO™ Legionella pneumophila IFA Test Kit to monitor the intracellular presence of L. pneumophila Paris (columns 2 and 4) and Olda (columns 1 and 3) within the Acanthamoeba strains SPA08 (columns 1 and 2) and 1BU (columns 3 and 4). The presence of L. pneumophila strains was determined at different time points: just after the co-culture preparation (T0) and at 18 h, 24 h and 48 h (T18, T24 and T36, respectively). Pictures were taken at 1000× magnification, scale bar = 9.2 μm