Fig. 2From: Optimized methods for Legionella pneumophila release from its Acanthamoeba hostsEffect of the eight release treatments methods applied to the free-living Acanthamoeba strains and to the co-cultures of the two L. pneumophila strains and the two Acanthamoeba strains. Amoeba inactivation was determined using the MPN method on NNA plates. Data are presented as the means ± SD (columns and error bars)Back to article page