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Table 3 Strains and plasmids used in this work

From: Transcriptomic analysis of a classical model of carbon catabolite regulation in Streptomyces coelicolor

Strains/plasmids Characteristics Reference
S. coelicolor M145 SCP1 SCP2 [61]
S. coelicolorglk SCP1 SCP2 glk::aacc4 This work
S. coelicolor Zm S. coelicolorglk, harboring the plasmid pUNAMZm This work
S. coelicolor 702 S. coelicolorglk, harboring the plasmid pIJ702 This work
E. coli ET12567 F-dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44 [62]
E. coli DH5α Invitrogen
Plasmids
 pIJ702 Derived from pIJ101 with an estimated copy number of approximately 50 [16]; John Innes Centre
 pIJ2442 Derivative of pUC19 containing the1.7 pb PstI-XbaI Z. mobilis glk fragment [16]; John Innes Centre
 pUNAM Zm Plasmid pIJ702 harboring the 1.7 pb PstI-XbaI Z. mobilis glk fragment from pIJ2442 This work