Fig. 3

Insertion inactivation of pefC in GAS leads to impaired growth and heme hypersensitivity. a Growth of the NZ131 (WT), ZE4951 (Mutant), ZE4951/pANKITA5b (Complement), and ZE4951/pKSM201 (Empty vector) strains in THYB. Fresh media were inoculated with GAS cells (OD_{600 nm} = 0.05) and the cultures were grown statically at 37 °C. Cell growth was monitored colorimetrically and expressed in Klett units. b-c: Growth of ZE4951/pANKITA5b (Complement) and ZE4951/pKSM201 (Empty vector) strains in THYB containing varying heme concentration. The same as with A, only that heme was added to the culture at the early logarithmic phase in final concentration of: b 1 μM; c 5 μM; and d 10 μM. The data are representative of at least two independent experiments