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Fig. 1 | BMC Microbiology

Fig. 1

From: The GAS PefCD exporter is a MDR system that confers resistance to heme and structurally diverse compounds

Fig. 1

The pefRCD genes entail an operon prevalent in human and zoonotic streptococci. a The genomic arrangement and occurrence of the pefRCD genes in GAS (MGAS5005 strain) and GBS (NEM316 strain) is also conserved among members of the pyogenic cluster namely, S. equi (4047 strain), S. uberis (0140 J strain), and S. iniae (ISET0901). Additionally, this system is found in S. suis (JS14 strain). These PefCD homologs are referred to as SatAB in the literature [37]. The pef operon consists of a MarR-like transcriptional regulator (locus tag for strains tested: Spy_0195, gbs1402, SEQ_0290, SUB_1690, SSUJS14_1996, and DQ08_09375) controlling an adjacent ABC-type efflux system (Spy_0196-97, gbs1401-00, SEQ_0292-93, SUB_1689-88, SSUJS14_1995-94, and DQ08_09370-65). The neighboring genes upstream and downstream from the pef operon consist of lipid metabolism (gpsA) and unknown function, respectively. b The GAS pefRCD genes are co-transcribed. RT-PCR analysis was preformed with 0.8 μg RNA extracted from NZ131 strain. cDNA synthesized by use of the pefD antisense primer (ZE650), was amplified by PCR with gene specific primers and fractionated on 1.2 % agarose gel (lanes 2, 5, and 8). PCR was also conducted using genomic DNA (lanes 3, 6, and 9) or RNA (without RT reaction, lanes 4, 7, and 10) as templates. Fragments from the following genes were amplified: pefR, lanes 2, 3 and 4; pefC, lanes 5, 6 and 7; and pefD, lanes 8, 9, and 10. The lines above the gene diagram in Figure-1A depict the amplified regions and the primer sets used in the PCR are included on top. The sizes of molecular mass standards are indicated to the left of the gel

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