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Fig. 5 | BMC Microbiology

Fig. 5

From: Influence of platelet-activating factor receptor (PAFR) on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortus

Fig. 5

PAFR activation plays a critical role in intensifying F-actin polymerization for phagocytosis of B. abortus. RAW 264.7 cells were pretreated for 5 min with PAF (200 nM) followed by infection with B. abortus for 5 (a), 15 (b), or 30 (c) min, and then cells were subjected to FACS analysis for F-actin content. (d) The quantitative analysis results of experiment in (a-d). The average F-actin content of a population was expressed as the mean of the fluorescence intensity. Data represent the mean ± SD of triplicate trials from three independent experiments. Statistically significant differences from the untreated samples are indicated by asterisks (*, P < 0.05; **, P < 0.01)

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BMC Microbiology

ISSN: 1471-2180

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