Fig. 4

PAFR cooperates with JAK2 signaling for phagocytosis of B. abortus. RAW 264.7 cells were pretreated with or without PAF (200 nM) for 5 min or CV3988 (1 μM) for 1 h prior to infection; the cells were then infected with Alexa Fluor 405-labeled B. abortus (blue) for the indicated times. To observe redistribution of F-actin polymerization and activated JAK2, the cells were fixed and stained with both rhodamine-conjugated phalloidin for F-actin (red) and FITC-labeled phospho-JAK2 antibody (green) immediately following a 10 min infection. The merged images are also shown and all scale bars in images represent 10 μm. All results are representatives of three separate experiments