Skip to main content
Fig. 2 | BMC Microbiology

Fig. 2

From: Influence of platelet-activating factor receptor (PAFR) on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortus

Fig. 2

The stimulation of PAFR positively affects uptake of B. abortus into macrophages. (a) RAW 264.7 cells were pretreated for 5 min with serial concentrations (0–200 nM) of PAF, a PAFR agonist, followed by infection with B. abortus for the indicated times. (b) Cells were pretreated for 1 h with a serial concentration (0–2 μM) of CV3988, a PAFR antagonist, and then infected as described in (a). Bacterial internalization efficiency was determined by evaluating the protection of internalized bacteria from gentamicin treatment. All data represent the mean ± SD of triplicate trials from three independent experiments. Statistically significant differences from the untreated samples are indicated by asterisks (*, P < 0.05; **, P < 0.01; ***, P < 0.001)

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us