Table 1 Solid media type influences isolation of Gram-negative skin microbiota. (Upper) Growth results from initial swab collected from participant on various plates and at either 32 or 37 °C using 4-quadrant streaking system. (Lower) Growth yields after inoculum isolated on R2A was re-plated under indicated conditions. Increasing amounts of bacterial growth are semi-quantitatively indicated as none (--), or as apparent only in the 1st quadrant (+), or into the 2nd (++) or 3rd (+++) streaked quadrant at 72 h. Data are representative of three independent experiments for each species. Each species represented by 1–3 clinical isolates
| Â | From Initial Swab | Â | Â | Â | Â | Â | Â | Â | ||
| Â | Blood Agar | BHI | Â | Chocolate | MacConkey | R2A | Â | |||
Bacteria | 32 | 37 | 32 | 37 | 32 | 37 | 32 | 37 | 32 | 37 |
R. mucosa | -- | -- | -- | -- | -- | -- | -- | -- | +++ | + |
P. septica | -- | -- | -- | -- | -- | -- | -- | -- | +++ | + |
M. osloensis | -- | -- | -- | -- | -- | -- | -- | -- | +++ | + |
P. aeruginosa | ++ | -- | -- | -- | ++ | + | ++ | + | +++ | + |
P. oryhabiztans | + | -- | -- | -- | ++ | + | -- | -- | +++ | + |
P. luteola | + | -- | -- | -- | ++ | + | -- | -- | +++ | + |
| Â | After Isolation | Â | Â | Â | Â | Â | Â | Â | Â | |
| Â | Blood Agar | BHI | Â | Chocolate | MacConkey | R2A | Â | |||
Bacteria | 32 | 37 | 32 | 37 | 32 | 37 | 32 | 37 | 32 | 37 |
R. mucosa | ++ | ++ | + | + | -- | -- | -- | -- | +++ | ++ |
P. septica | ++ | ++ | ++ | ++ | ++ | ++ | + | + | +++ | ++ |
M. osloensis | ++ | ++ | ++ | ++ | + | + | -- | -- | +++ | ++ |
P. aeruginosa | ++ | -- | ++ | ++ | ++ | + | + | ++ | +++ | ++ |
P. oryhabiztans | ++ | -- | ++ | ++ | ++ | + | ++ | + | +++ | ++ |
P. luteola | ++ | ++ | ++ | ++ | ++ | + | ++ | + | +++ | ++ |