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Table 1 Solid media type influences isolation of Gram-negative skin microbiota. (Upper) Growth results from initial swab collected from participant on various plates and at either 32 or 37 °C using 4-quadrant streaking system. (Lower) Growth yields after inoculum isolated on R2A was re-plated under indicated conditions. Increasing amounts of bacterial growth are semi-quantitatively indicated as none (--), or as apparent only in the 1st quadrant (+), or into the 2nd (++) or 3rd (+++) streaked quadrant at 72 h. Data are representative of three independent experiments for each species. Each species represented by 1–3 clinical isolates

From: A method for culturing Gram-negative skin microbiota

  From Initial Swab        
  Blood Agar BHI   Chocolate MacConkey R2A  
Bacteria 32 37 32 37 32 37 32 37 32 37
R. mucosa -- -- -- -- -- -- -- -- +++ +
P. septica -- -- -- -- -- -- -- -- +++ +
M. osloensis -- -- -- -- -- -- -- -- +++ +
P. aeruginosa ++ -- -- -- ++ + ++ + +++ +
P. oryhabiztans + -- -- -- ++ + -- -- +++ +
P. luteola + -- -- -- ++ + -- -- +++ +
  After Isolation         
  Blood Agar BHI   Chocolate MacConkey R2A  
Bacteria 32 37 32 37 32 37 32 37 32 37
R. mucosa ++ ++ + + -- -- -- -- +++ ++
P. septica ++ ++ ++ ++ ++ ++ + + +++ ++
M. osloensis ++ ++ ++ ++ + + -- -- +++ ++
P. aeruginosa ++ -- ++ ++ ++ + + ++ +++ ++
P. oryhabiztans ++ -- ++ ++ ++ + ++ + +++ ++
P. luteola ++ ++ ++ ++ ++ + ++ + +++ ++