Fig. 3

Growth curves for select commensal Gram-negative and Staphylococcus species. A single colony of bacteria was added to liquid media at time zero for all indicated isolates. Colony forming units (CFU) assessed by serial dilutions at indicated time points. a Growth performed at 32 °C, in R2A broth for both CGN and staphylococcal species for direct comparison of growth kinetics. b Comparison of optimized growth kinetics; CGN cultured at 32 °C in R2A broth, Staphylococcus species cultured at 37 °C in TSB. Data are representative of two or more independent experiments and depicted as mean + SD. Each species represented by 1–3 clinical isolates