Table 2 Primers for PCR amplification of the connection region of integron and ISCR1

A

I

aadA2 + bla _CTX-9F

CGTTGCCTTGGTAGGTCC

II

aadA2 + bla _CTX-9 up R

TTGCTTGCCATAGTCATCTT

IX

aadA2 + bla _CTX-9 down F

CCCCAAGGAGCCCATTC

X

aadA2 + bla _CTX-9R

GGTATTCAGCGTAGGTTCAGT

B

I

aadA2 + qnrA1 F

GTTGTCCCGCATTTGGT

II

aadA2 + qnrA1 up R

GGTTGAGCGAGAAGGTTTT

IX

aadA2 + qnrA1 down F

GCGTGAGCTGCCACCAGAA

X

aadA2 + qnrA1 R

TCTTATGGCTGACTTGATTGTAG

C

I

aadA1 + bla _DHA-1 F

ATCTGGCTATCTTGCTGAC

II

aadA1 + bla _DHA-1 up R

TTCCGAGAAGGTGATTGC

IX

aadA1 + bla _DHA-1 down F

CCAACACTGCTCAACACT

X

aadA1 + bla _DHA-1 R

GGTGGCGATTGTGATTCT

D

I

dfrA25+ sapA F

ACGAAGCGATGGGTAGA

II

dfrA25+ sapA up R

AGCCCTCACGAGTTGTTAT

IX

dfrA25+ sapA down F

CAAGAAGCCCGACAAAT

X

dfrA25+ sapA R

TGGGAGGTGCTGGATAA

E

I

aadA2+ sapA F

CGTTGCCTTGGTAGGTC

II

aadA2+ sapA up R

AACCGCACAATCTCGTC

IX

aadA2+ sapA down F

CGCTGCTGATAGACGAAG

X

aadA2+ sapA R

TGGGAGGTGCTGGATAA

F

I

aadA16+ sapA F

GTTGTTCCTTGGCGTTATC

II

aadA16+ sapA up R

TCAGCAATATCGGGATAGAG

IX

aadA16+ sapA down F

AGACGATACGCTGACTCA

X

aadA16+ sapA R

ATGACCGACTGCTTGATG

G

I

dfrA27 + short chain F

GCAATGAGGGAGCTAAAGA

II

dfrA27 + short chain up R

TTGGGTTCAGGGTGCTAT

IX

dfrA27 + short chain down F

CAAGAAGCCCGACAAATC

X

dfrA27 + short chain R

TTCACGAGCATAGGCAATA

H

I

aacA4 + bla _PER F

CCCGAGGTCACCAAGA

II

aacA4 + bla _PERup R

GCACCATCCCACATAAGA

IX

aacA4 + bla _PERdown F

AAGAGGGCGAAGACGA

X

aacA4 + bla _PER R

TCCATCAGGCAACAGAAT

I

I

aadA5 + bla _PER F

ACTGGTCTCATTGCTCCTA

II

aadA5 + bla _PER up R

CGAAGAACCGCACAATCT

IX

aadA5 + bla _PER down F

CAACACTGCTCAACACTG

X

aadA5 + bla _PER R

ATTGGTTCGGCTTGACTC

J

I

aadA24 + bla _PER F

CATCATTCCGTGGCGTTA

II

aadA24 + bla _PER up R

GACACCGAGACCAATAGC

IX

aadA24 + bla _PER down F

AATCCAACACTGCTCAACA

X

aadA24 + bla _PER R

CATCATTCCGTGGCGTTA

K

I

arr-3 + qnrA1 F

GGTAATCCAACACAGTCCTA

II

arr-3 + qnrA1 up R

GTCCGCCTCAGCAATATC

IX

arr-3 + qnrA1 down F

TCCAACACTGCTCAACAC

X

arr-3 + qnrA1 R

CCAGAGTATCCGCAATCC

L

I

bla _PSE-1 + dfrA10 F

TTATGGCGGCGTTAGATG

II

bla _PSE-1 + dfrA10 up R

CGAGACCAATAGCGGAAG

IX

bla _PSE-1 + dfrA10 down F

ATATTGAAGTCTGCGAACAC

X

bla _PSE-1 + dfrA10 R

CGTGCTCTGTGATAGTTGA

-

III

common 1 791 F

TATTGCTGAGGCGGACTG

IV

common 1 791 R

CATTGGAGGAGGTCGTTG

V

common 2 1054 F

GGCTTCCGCTATTGGTC

VI

common 2 1054 R

TTGCTTGCCATAGTCATCTT

VII

common 3 1727 F

TCGCCCACTCAAACAAA

VIII

common 3 1727 R

GCTCCTCATCCGAAGTATCT

A

-

CCTGTCGGTGTTGCTTAT

B

-

GTTGCTTGCCATAGTCATC