Fig. 2

Visualisation of the antibacterial effect of CORM-2 and cefotaxime on established biofilm. Effect of CORM-2 and cefotaxime on bacterial viability within an established biofilm (biofilm grown for 24 h) evaluated by a live/dead viability staining assay using confocal microscopy. Live bacteria with intact cell membrane are stained green (SYTO9) and dead bacteria with damaged cell membrane are stained red (propidium iodine). Photographs show representative areas from the chamber slides; from left to right isolate UPEC isolate 2, ESBL isolate 1 and K-12 strain TG1 and from top-down a controls, b 24 h CORM-2 (500 μM), c 24 h cefotaxime (0.512 μg/ml). Scale bar = 10 μm. Representative photographs from three independent experiments are shown