Fig. 4

Detection of A. minutissimum biofilms with a plate reader before and after staining with CV. Absorbance was measured at wavelengths 630, 650 and 750 nm before staining the biofilms with crystal violet (CV), and at 580 nm after the staining. Negative, axenic controls; biofilm-positive, xenic controls; co-culture of axenic A. minutissimum and Bacteroidetes strain 32; N = 48 to 64 wells per culture type, spread across 4 plates, across 2 independent experiments