Table 2 Oligonucleotides used in this study
No | Name | Sequence |
|---|---|---|
1 | klcApRA3L | GCGCATGCGGGAGCGTGATCGTTACGGT |
2 | klcApRA3R | GCGGATCCATTGCAGCCATACGGCGAGG |
3 | klcAsmaF | GGCCCGGGTGCTCGTCTCGTCGGTCTG |
4 | xylEPscR | GGACATGTCATCTGCACAATCTCTGCA |
5 | KBRK2Apa | CCGAATTCGGGCCCGAAGATGGAGATTTCCCAATGACTGC |
6 | KBRK2Sal | CCGTCGACCGCTGTCTTTGGGGATCAGCCCTC |
7 | lacOMCS1 | CATGGAATTGTGAGCGCTCACAATTTCAGATCTGAATTCTACGTACTCGAGCTCGGAATTGTGAGCGCTCACAATTTCA |
8 | lacOMCS2 | CGCGTGAAATTGTGAGCGCTCACAATTCCGAGCTCGAGTACGTAGAATTCAGATCTGAAATTGTGAGCGCTCACAATTC |
9 | parSbg2a | GATCGGTTGCTTGTTCCACGTGGAACAAGGCCG |
10 | parSbg2b | GATCCGGCCTTGTTCCACGTGGAACAAGCAACC |
11 | NrulacIF | GGTCGCGACTGAATCCGGTGAGAATGG |
12 | PvulacIR | CGCGATCGATAAGCTTGCAATTCGCG |
13 | oriTRK2Fs | ACGGTCGACACATGTCTGGTTGGCTTGGTTTCATC |
14 | oriTRK2Rs | CGGAATTCACATGTTTGCCAAAGGGTTCGTGTAG |
15 | oriTRA3F | CGCGTCGACACATGTTTTAGCACAAGCGGCGGCAG |
16 | oriTRA3R | CGGAATTCACATGTAGTTAGGGGAAGCCGACGAG |
17 | Nco | ACCATGGTCATG |
| Â | Primers used in site-directed mutagenesis | |
18 | muttrfA RK2 | GTCCTTGAGAAAGGAGACgtc gGTTTAGCTA |
19 | muttrfA RK2 | CCAATGTTTAGCTAAACc gacGTCTCCTTTC |
20 | xhoF8180 | CGGGCCGTCGGCTCGaGCATCATATCGAC |
21 | xhoR8180 | CGATATGATGCtCGAGCCGACGGCCCGC |
22 | apaF9950 | CTTTCTGGTTGGCCGgGcCCAAAGTTTTtATCGTTTGGTTTCC |
23 | apaR9950 | GAAACCAAACGATaAAAACTTTGGgCcCGGCCAACCAGAAAGGC |
24 | Ppu618F | CGCTGCAGAGGCCAGACCCCGTGAAATT |
25 | Ppu618R | GCAAGCTTGGTCAGCATAGTCCACCTCA |
| Â | Primers used for Real Time qPCR | |
26 | galKF | ATGATCTTTCTTGCCGAGCG |
27 | galKR | AGCAGCTTTATCATCTGCCGC |
28 | trfAF | GTGAAGATCACCTACACCGGC |
29 | trfAR | TGGCAAAGCTCGTAGAACGTG |
30 | repBRA3F | CATCGAGAAGCAAAAGGCG |
31 | repBRA3R | CCAACTTGCGTAGGTCTTCCAG |