Fig. 4
Functionality of test vectors in various bacterial species. a P. aeruginosa PAO1161 was transformed with pESB32 and pESB34 (korA-incC-korB-orf11-parS). Three transformants were grown for 40 generations without selection. The retention of plasmid was estimated by percentage of blue colonies on L agar plates with X-gal. The results shown are average from three experiments with standard deviation. b A. tumefaciens LBA1010R RifR was conjugated with E. coli S17-1(pESB32) and S17-1(pESB34) donor strains. Obtained transconjugants were selected on L agar supplemented with chloramphenicol and rifampicin. Three independent transconjugants were grown for 40 generations without selection. The retention of plasmid was estimated by percentage of blue colonies on L agar plates with X-gal. The results shown are average from three experiments with standard deviation