Table 4 Bacterial and yeast strains, and plasmids used in this study
Strain or plasmid | Relevant genotype and/or characteristics | Reference or source |
|---|---|---|
Agrobacterium tumefaciens strains | Â | |
 C58C1 | Ti plasmid-less C58rif; Rifr | Our collection |
 EHA105 | C58 containing pTiEHA105 (T-DNA deletion) | [50] |
Escherichia coli strain | Â | |
 HB101 | F -, recA13, proA2 | [51] |
 SURE | F′[lacIq lacZΔM15] lac recB recJ sbcC umuC::Tn5(KanR) uvrC | Stratagene |
Saccharomyces cerevisiae strains | Â | |
 BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | [52] |
 Mutants derived from BY4742 | The yeast genome deletion project | |
 BY4742floxU | BY4742 with loxP::URA3::loxP inserted in pda1 | This study |
 BY4742 mutants with loxP::URA3::loxP | This study | |
Plasmids | Â | Â |
 pBIN19 | Binary vector with an artifical T-DNA (LB, P nos :nptII, lacZ’ with MCS, RB) and nptIII; Kmr | [44] |
 pYAC4 | Yeast artificial chromosome; HIS3, (Tel, TRP1, ARS1, CEN4, URA3, Tel); Ampr (Carr) | [45] |
 pYAC4-B | BamHI-digested and ligated pYAC4; (Tel, TRP1, ARS1, CEN4, URA3, Tel); Ampr (Carr) | This study |
 pYAC4-X | XhoI-digested and ligated pYAC4; (TRP1, ARS1, CEN4, URA3); Ampr (Carr) | This study |
 pBY1 | pBIN19 containing (Tel, TRP1, ARS1, CEN4, URA3, Tel) at MCS in T-DNA; Kmr and Ampr (Carr) | [16] |
 pBIN19Δ | pBIN19 having nothing but 33 nucleotides including BamHI cutting site between LB and RB A PCR product. | This study |
 pBINU1 | pBIN19Δ harboring URA3 at BamHI site in T-DNA; Kmr | This study |
 pBINU2 | pBINU1 lacking the 52-bp homology segment | This study |
 pBYM3 | pBIN19Δ harboring (TRP1, ARS1, CEN4, URA3) at BamHI site in T-DNA; Kmr and Ampr (Carr) | This study |
 pBYM4 | pBIN19Δ harboring (Tel, TRP1, ARS1, CEN4, URA3, Tel) at BamHI site in T-DNA; Kmr and Ampr (Carr) | This study |
 pSDM3013 | pBIN19 with pda1::loxP::URA3::loxP::pda1at MCS in T-DNA; Kmr | [2] |
 pRi1724-S3CE2 | pRi1724-S3 containing P virE ::virE1::cre::virE2 fusion in place of a region starting from riorf135 to riorf144; Gmr | [16] |
 pRS313 | HIS3, ARSH4/CEN6 and Amp r (Car r) | [53] |
 pSRS2 | SRS2 in pRS313 | This study |
 pRAD52 | RAD52 in pRS313 | This study |
 pSMI1 | SMI1 in pRS313 | This study |
 pERG28 | ERG28 in pRS313 | This study |
 pRH210 | oriV, oriT incP, mob incP, tra incP and Amp r | [54] |
 pAY205 | ARS1, TRP1, URA3, oriV incQ, oriT incQ, mob incQ and Km r | [54] |