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Table 3 Comparison of AMT efficiency of wild type strain between replicating and integrative T-DNA

From: DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNA

Donor strain T-DNA features Co-cultivation period AMT efficiency Relative efficiency (%/pBY1)
EHA105 (pBY1) Tel a , ARS/CEN, URA3, Tel a 1 day (1.9 ± 0.1) × 10-3 (100)
EHA105 (pSDM3013) pda1 : : URA3 b 3 days (2.5 ± 0.4) × 10-5 ** 1.4
EHA105 (pBINU2) URA3 6 days (4.0 ± 0.1) × 10-7 ** 0.02
EHA105 (pBYM4) Tel c , ARS/CEN, URA3, Tel c 1 day (9.0 ± 1.9) × 10-3 ** 483
EHA105 (pBYM3) ARS/CEN, URA3 1 day (2.0 ± 0.3) × 10-4 ** 11
  1. a The telomere sequence is located inside T-DNA
  2. b URA3 gene is contained in PDA1 locus
  3. c The telomere sequence is located in close proximity to T-DNA terminal end
  4. Each value is the average of three experiments. Differences were statistically significant compared to EHA105 (pBY1) by Student’s t-test. ** p <0.01