DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNA

Table 2 Effect of different recipient cell abundance on AMT efficiency

No. of input donor (cells/ml)	No. of input recipient (cells/ml)	donor : recipient input ratio	AMT efficiency^a (×10^-3)	Relative efficiency (%)
4 × 10¹⁰	2.5 × 10⁵	160000 : 1	6.7 ± 1.7	159
4 × 10¹⁰	1 × 10⁶	40000 : 1	4.2 ± 0.4	(100)
4 × 10¹⁰	4 × 10⁶	10000 : 1	0.037 ± 0.006**	0.9

^a The wild type yeast cells were mixed with the donor EHA105 (pBY1) cells, and then cocultivated for 24 h. AMT efficiency is the average of three experiments
Differences were statistically significant compared to donor:recipient input ratio=40000:1 by Student’s t-test. ** p <0.01

ISSN: 1471-2180