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Fig. 8 | BMC Microbiology

Fig. 8

From: DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNA

Fig. 8

Alignment of the junction sequences in the T-DNA circles of yeast AMT transformants. DNA circles from the transformants were classified into three types. a T-DNA with perfect RB-LB borders junctions, b whole pBY1 plasmid, and c pBY1 with a 9310-bp deletion via intra-plasmid recombination. Nucleotide sequences derived from the RB are shaded in red, while sequences from the LB are shaded by blue. The recombination region between the two 378-bp homology sequences (pMB1 ori) is indicated in green, whose internal sequence is abbreviated. The schematic diagram demonstrates the recombination sites in pBY1

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BMC Microbiology

ISSN: 1471-2180

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