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Fig. 4 | BMC Microbiology

Fig. 4

From: DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNA

Fig. 4

Efficiency of VirE2 protein transport from Agrobacterium into the yeast mutants. The yeast mutant strains that contained lox::URA3::lox at the PDA1 locus were co-cultivated with Agrobacterium C58C1 harboring pRi1724-S3CE2 (cre::virE2), and then Uraˉ transformants were selected on SC medium supplemented with 0.1 % 5-fluoroorotic acid and 200 μg/ml cefotaxime. The relative efficiency was calculated by dividing by the efficiency of the wild-type strain. Error bars indicate the standard deviations of triplicate assays. Differences were statistically significant compared to the wild type strain by Student’s t-test. *p <0.05, **p <0.01

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