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Fig. 3 | BMC Microbiology

Fig. 3

From: DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNA

Fig. 3

Effect of T-DNA type and co-cultivation conditions on AMT efficiency. a Yeast cells were co-cultivated with Agrobacterium EHA105 harboring a binary plasmid shown in each column. The co-cultivation time was changed depending on each donor: EHA105 (pBY1), 1 day; EHA105 (pSDM3013), 3 days; EHA105 (pBINU2), 6 days. b Yeast mutant cells were co-cultivated with EHA105 harboring pBY1 for the durations shown the figure. Relative efficiency was calculated by dividing the AMT efficiency by that of the wild-type strain. Error bars indicate the standard deviations of triplicate assays. Differences were statistically significant compared to the wild type strain by Student’s t-test. * p <0.05, ** p <0.01

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BMC Microbiology

ISSN: 1471-2180

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