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Fig. 2 | BMC Microbiology

Fig. 2

From: DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNA

Fig. 2

Lithium acetate transformation frequencies of yeast mutant strains. a Yeast cells were transformed with either 0.5 μg pYAC4-B linearized by BamHI treatment or 0.5 μg of circular pYAC4-B. Transformation frequency was defined as the number of transformants per μg DNA divided by the number of viable cells after the Lithium acetate and heat-shock treatments. b Yeast cells were transformed with 0.3 μg of pYAC4-B or telomere-less plasmid pYAC4-X. Relative transformation frequency was expressed as the ratio of the transformation frequency obtained with linearized plasmid divided by that using circular plasmid. Error bars indicate the standard deviations of triplicate assays. Differences were statistically significant compared to the wild type strain by Student’s t-test. **p <0.01

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BMC Microbiology

ISSN: 1471-2180

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