Skip to main content

Table 1 Discriminatory potential of the loop-encoding DNA sequence compared to 16S (Vibrio) or 18S rRNA gene (Aspergillus)

From: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme

 

16S rRNA gene

CCA-adding enzyme loop

Comparative potential

 

Length [bp]

Number of specific mutations

%

Length [bp]

Number of specific mutations

%

%/%

V. alginolyticus

850

9

1.1

75

10

13.3

12.1

V. parahaemolyticus

850

1

0.1

75

8

10.7

107.0

V. vulnificus

850

42

4.9

75

21

28.0

5.7

Interspecific similarity

94.0–98.8 %

65.3–82.7 %

 
 

18S rRNA gene

    

A. fumigatus

850

3

0.4

63

9

14.3

35.8

A. niger

850

1

0.1

63

10

15.9

159.0

A. terreus

850

4

0.5

63

7

11.1

22.2

Interspecific similarity

99.2–99.5 %

74.6–79.4 %

 
  1. The discriminatory potential is given as percentage sequence differences. The comparative potential is represented by the ratio of the discriminatory potentials. Interspecific similarities were calculated based on pairwise alignments in Fig. 1b. For the 16/18S rRNA gene, the first 850 positions were taken into account, whereas the flexible loop sequence was analyzed in full length. Although the loop sequence represents only 7.4 to 8.8 % in length compared to the analyzed rRNA gene region, the percentage values of mutations show a substantially higher discrimination potential of this sequence. The specific mutations within the indicated genes are unique and characteristic for the individual species