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Fig. 1 | BMC Microbiology

Fig. 1

From: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme

Fig. 1

A highly variable loop element in CCA-adding enzymes. a. The flexible loop (green) is located between two highly conserved motifs A and B of the catalytic core. At the DNA level, this allows for the annealing of a genus-specific forward primer fV (Vibrio) or fA (Aspergillus), indicated by the arrow. b. Sequence alignment of the individual loop regions of V. alginolyticus, V. parahaemolyticus, V. vulnificus, A. fumigatus, A. niger, and A. terreus. Red characters indicate species-specific point mutations. Arrows indicate the hybridizing regions of the reverse primers. Primer combinations used in the amplification reactions are shown on the right

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