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Table 1 Bacterial strains and plasmids used in this study

From: Identification and functional analysis of the L-ascorbate-specific enzyme II complex of the phosphotransferase system in Streptococcus mutans

Strains or plasmids

Relevant characteristics

Source or reference

Strains

  S. mutans UA159

Wild-type, serotype c

Ajdić et al., (2002) [4]

  S. mutans ptxA −

UA159 derivative, △ptxA −, Sper

This study

  S. mutans ptxB −

UA159 derivative, △ptxB −, Sper

This study

  S. mutans ptxAB −

UA159 derivative, △ptxA − and △ptxB −, Sper

This study

  S. mutans CptxA −

S. mutans ptxA − carrying pDL278:ptxA, Sper

This study

  S. mutans CptxB −

S. mutans ptxB − carrying pDL278:ptxB, Sper

This study

  S. mutans CptxAB −

S. mutans ptxAB − carrying pDL278:ptxAB, Sper

This study

Plasmids

 pFW5

Commercial cloning vector, Sper

Podbielski A et al., (1996) [52]

 pDL278

Shuttle vector, Sper

LeBlanc & Lee (1991) [53]

 pDL278:ptxA

Shuttle vector carrying ptxA, Sper

This study

 pDL278:ptxB

Shuttle vector carrying ptxB, Sper

This study

 pDL278:ptxAB

Shuttle vector carrying ptxA and ptxB, Sper

This study

  1. Spe r spectinomycin resistance