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Fig. 4 | BMC Microbiology

Fig. 4

From: Identification and functional analysis of the L-ascorbate-specific enzyme II complex of the phosphotransferase system in Streptococcus mutans

Fig. 4

Transcription evaluation of ptxA, ptxB and their operon. a PCR analysis of ptxA, ptxB and adjacent genes with specific primers that span the sequences SMU.268 to sgaT, sgaT to ptxB, ptxB to ptxA, ptxA to SMU.273, SMU.273 to SMU.274, SMU.274 to SMU.275 and SMU.275 to SMU.277 by using cDNA. The letters a-g correspond to the amplified regions illustrated above the agarose gel. Lanes: M1, 100 bp DNA Ladder; DNA, chromosomal DNA of UA159; cDNA, cDNA of UA159; M2, 1 kb DNA Ladder. b Quantitative real-time PCR (qRT-PCR) analysis of the influence of L-ascorbate on the transcription levels of ptxA, ptxB and adjacent genes. The results are presented as relative mRNA expression. Significant differences are indicated by **P < 0.01. c Expression of ptxA gene in wild-type UA159 and ptxB strain evaluated by qRT-PCR. There was a statistically significant difference between these two strains (**P < 0.01). The qRT-PCR results presented here are the average of three independent experiments performed in triplicate

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