Fig. 6

Analysis of the immunological properties of isolated flagellins. a Filamentous flagellins (10 pmol/ml) were added to Caco-2 cell cultures and released IL-8 was quantified by ELISA. Values are mean + SE (n = 4). Statistical significance was also shown (P < 0.05). b Caco-2 cells were stimulated with native (filamentous) or urea-treated (depolymerized) flagellins at 100 pmol/ml. Values are mean + SE (n = 3). c Alignment of amino acid sequences of major flagellins from pathogenic bacteria and lactobacilli (FliC2). Conserved residues which were reported to be important for TLR5 recognition by Smith et. al. are highlighted. Boxed letters are different from the conserved amino acid residues. Underlines represent polymorphic amino acids between FliC1 and FliC2: A and K are K and A in FliC1. References: [7, 17, 18]