Differential expression of Spiroplasma citri surface protein genes in the plant and insect hosts

BMC Microbiology

Table 1 List of selected genes to be tested as reference genes in the present study

Name	GenBank accession	Symbol	Function	Primer sequences (5′ to 3′) foward/reverse	Amplicon length
Putative chromosomal replication initiator protein dnaA	SPICI01B_001	dnaA	Replication	ATGAGTAAATCACGAGTTAG TCTTTGCCACCGAACTCTG	116
Dna gyrase subunit b protein	SPICI01B_003	gyrB	Topoisomerase	GGAGATTCTGCTGGTGGAAGTG TCTTTAATACCTGCTCCTAATGCG	167
Dna gyrase subunit a protein	SPICI01B_004	gyrA	Topoisomerase	TTCGCCAAACAGGGAAAGTAG CTCCAGTAGCATCATTAGCAATTC	195
Dna-directed rna polymerase beta chain protein	SPICI01B_073	rpoB	Transcription	TGTGCCATTAGTGCGTCAAG CATCTTCTGATACTAAGCGTTCTG	179
Hypothetical chromosome replication initiation and membrane attachment protein	SPICI03_040	dnaB	Replication	AATTACCAATTTCCGCAATTGC TTGTTTGTCTTCTTGATTATTAAC	131
50s ribosomal protein L3	SPICI03_102	rplC	ribosomal protein L3	AATGCCTGGACATATGGGAAC GCATCAACAACTACAACTGG	252
Spiralin lipoprotein	SPICI04_139	spi	Lipoprotein	ACAACGAAGGTACATCATTAACAAC TTTGCTGGAGTAATTTGAACATAAAC	80
Pyruvate kinase protein	SPICI04_141	pyK	Glycolysis	GGGAATTATTAAAAACAATTTC TTGCCACTTCACAAATTGC	171
Fibril protein	SPICI12_006	fib	Cytoskeleton Structure	TAAGCATGATACAGGAGATACAAC TGCCCATATTTATCAACCATTTCC	246
Cell shape-determining protein mreb1	SPICI13_009	mreB1	Cell morphogenesis	AGGAACAACAGACATTGCGG TCTCTAGCCCATATTGAGAAC	125
16S rRNA	ND	16S	30S ribosome subunit RNA component	CAAATCCTGGAGCTCAACTC GCGTAGACTACTAGGGTATC	204

ISSN: 1471-2180