Fig. 2From: Differential expression of Spiroplasma citri surface protein genes in the plant and insect hostsComparison of the relative expression levels of S. citri lipoprotein genes (-ΔΔCT) in leafhopper-infected plants (LIP) and in insects. The -ΔΔCT value calculated for each host was obtained by subtracting the respective ΔCT of the target gene in the calibrator sample corresponding to axenic medium from those of the target gene in the host. Positive -ΔΔCT value indicate an up-regulation of the target gene, while a negative value indicates its down-regulation. Experiments were carried out on three independent biological replicates, each consisting of three replicate reactions. A change in │ΔΔCT│ in host versus axenic medium was considered as significant if superior to 1 (either above (up-regulated genes) or below (down-regulated) the dashed lines). Asterisks indicate genes, for which the expression level is significantly different in insects and in LIP as determined using the Student’s t test (P < 0.05, │ΔΔCT│ > 1 in at least one host). Grey bars indicate -ΔΔCT measured in LIP and the black bars the -ΔΔCT in infected insectsBack to article page