Table 3 Primers designed for genome walking in this study
From: Bioprospecting potential of halogenases from Arctic marine actinomycetes
Gene | Upstream primers (5´- 3´) | Downstream primers (5´- 3´) |
|---|---|---|
531Â F | 531USP1: GCTCCTTCAGCCAGAATTGATG | 531DSP1: GATTACGCCTATCATTTCGATGCCTC |
531USP2: ATCTTGCCGAAGCCGTGGATGT | 531DSP2: CGCATCGTCGCCGCCAACCGCC | |
531USP3: ATCTTGCCGAAGCCGTGGATGT | 531DSP3: ATGGCGACCTGTTCATCGACTGCTC | |
551Â F | 551USP1: GTGCTTGCGCTGGAACCAGTAGT | 551DSP1: GGCTGGGCGTGGAAGATCCCGATGC |
551USP2: CGAACGGGTGGTAGAAGTGGTCCGGC | 551DSP2: GCGAGTTCTGCGAGATGTGGGGGCTGG | |
551USP3: CATCTTGAAGCTGGCGTTGCACTCCCGCA | 551DSP3: GCCTGGGTGAAGAACGTGGTCAGCATCG | |
597Â F | 5GSP597-1: CCTTACGGGCGTGGTCCAGCA | 3GSP597-1: GCCCACGTACTGACACAGGCCCAT |
5GSP597-2: CCACCTGCGTCAGTGAACTCG | 3GSP597-2: GCCGCCTACGTCGTCGACTCCAGC | |
5GSP597-3: CCATGTAGGGCAGCAGAGACTC | 3GSP597-3: AGCGGAAAGATCGCCAAGGCATAC | |
604Â F | 604USP1: CGCTCACGGACCACGACGCCCTT | 604DSP1: GGTGCGCTTCGAGGACGAGAACGG |
604USP2: GAGGAGCATCTGGTCGAACTTCAT 604USP3: TGCCGCCCCGCTTCTTGACGAAC | 604DSP2: ACGATGGACTCCAGTCGCCGCTACTCGGACTTCTTCCAG 604DSP3: GACGCGGGCTGGTACTGGTACAT |