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Table 2 Strains and plasmids used in this study

From: The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri

Strains and plasmids Characteristics Reference or source
Strains
Escherichia coli   [50]
DH5α F- recA1 endA1 hsdR17 supE44 thi-1 gyrA96 real1 ∆ (argE-lacZYA) 169 Ø lazA ∆ M15 Promega
Xanthomonas citri subsp. citri
306 Syn. X. axonopodis pv. citri strain 306; wild-type, Rfr, Apr [25, 51]
hrpB (hrpB-) hrpB (XAC0293): pNPTS138 derivative This study
hrpB-p53hrpB (hrpB+) hrpB (XAC0293) contained in pUFR053, Gmr This study
Plasmids
pGEM T-Easy PCR cloning and sequencing vector, lacZ, Apr Promega
pNPTS138 Kmr, sacB, lacZα+ M. R. Alley, unpublished
pUFR053 IncW Mob+ mob (P) lacZα+ Par+, Cmr, Gmr, Kmr, shuttle vector [52]
pNPTS_hrpB hrpB (XAC0293) gene cloning on pNPTS138 This study
p53_hrpB hrpB (XAC0293) gene cloning on pUFR053 This study
  1. Apr, Cmr, Gmr, Kmr, and Rfr indicate resistance to ampicillin, chloromycetin, gentamicin, kanamycin and rifamycin, respectively