Strains and plasmids | Characteristics | Reference or source |
---|---|---|
Strains | ||
Escherichia coli | [50] | |
DH5α | F- recA1 endA1 hsdR17 supE44 thi-1 gyrA96 real1 ∆ (argE-lacZYA) 169 Ø lazA ∆ M15 | Promega |
Xanthomonas citri subsp. citri | ||
306 | Syn. X. axonopodis pv. citri strain 306; wild-type, Rfr, Apr | |
∆hrpB (hrpB-) | hrpB (XAC0293): pNPTS138 derivative | This study |
∆hrpB-p53hrpB (hrpB+) | hrpB (XAC0293) contained in pUFR053, Gmr | This study |
Plasmids | ||
pGEM T-Easy | PCR cloning and sequencing vector, lacZ, Apr | Promega |
pNPTS138 | Kmr, sacB, lacZα+ | M. R. Alley, unpublished |
pUFR053 | IncW Mob+ mob (P) lacZα+ Par+, Cmr, Gmr, Kmr, shuttle vector | [52] |
pNPTS_hrpB | hrpB (XAC0293) gene cloning on pNPTS138 | This study |
p53_hrpB | hrpB (XAC0293) gene cloning on pUFR053 | This study |