Fig. 7

Identification of the fimA (XAC3241) transcriptional start site in X. citri subsp. citri. The transcriptional start site was determined using 5′ RACE. a A specific PCR product was detected after amplification was performed using reverse-transcribed cDNA with gene-specific primers for the pilA gene and with the adapter primers SP2 and SP1 (Roche). b Sequencing of the PCR products showed that the nucleotide indicated by arrow is the transcription start site of fimA in X. citri. The +1 nucleotide is indicated with an arrow, and the −35 and −10 sequences are shown in bold and italic, respectively. ATG is indicated in red, and the specific primer used to amplify the fragment is shown in bold. The letters represent the hairpins that were identified in the RNA: a = 1^st hairpin, b = 2^nd hairpin and c = 3^rd hairpin